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Bio-Rad
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Genzyme
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Image Search Results
Journal: Virus Research
Article Title: Monitoring longitudinal immunological responses to bluetongue virus 17 in experimentally infected sheep
doi: 10.1016/j.virusres.2023.199246
Figure Lengend Snippet: Experimentally infected sheep exhibited dynamic immune responses over the study time course. Sheep peripheral blood was analyzed for specific cell subsets using flow cytometry and cell specific antibodies. Broader cell populations, as indicated by complete blood counts, show (A) lymphocytes, and (B) neutrophils cell counts. CBC data is shown as BTV-17 infected relative to mock (set to 100 %) for each timepoint. Specific cell populations were identified by flow cytometry (C) CD8+ T cells, (D) CD4+ T cells, the CD8+/CD4+ T cells ratio (E), (F) B cells, (G) and CD14+ monocytes were detected and evaluated. Data is shown as mock infected (green) and BTV-17 infected (red). To account for animal-to-animal variation, all data points were standardized to signal at day 0..
Article Snippet: The following anti-sheep antibodies were used according to manufacturer's specifications: CD8α (clone ST8, Cat# SHP2002, WSU), CD4 (clone S-17D, Cat #S-GT2002, WSU), CD72 (clone 2–104) ,
Techniques: Infection, Flow Cytometry
Journal: Virus Research
Article Title: Monitoring longitudinal immunological responses to bluetongue virus 17 in experimentally infected sheep
doi: 10.1016/j.virusres.2023.199246
Figure Lengend Snippet: Evaluation of terminal tissues of BTV infected sheep. RT-qPCR was performed on terminal lung (A) and spleen (B) tissue samples of three positively infected animals using BTV specific primers. Immune populations (CD8+ T , CD4+ T , B cells, and CD14+ monocytes were evaluated in lung (C–F), and spleen (G–J) of terminal collected tissues. (K, L) CXCL10 expression in terminal lung and spleen tissue of both mock and BTV-17 infected animals was determined by RT-qPCR using ovine CXCL10 specific primers.
Article Snippet: The following anti-sheep antibodies were used according to manufacturer's specifications: CD8α (clone ST8, Cat# SHP2002, WSU), CD4 (clone S-17D, Cat #S-GT2002, WSU), CD72 (clone 2–104) ,
Techniques: Infection, Quantitative RT-PCR, Expressing